Candida is a genus of yeasts and is the most common cause of fungal infections worldwide. The virulence of Candida isolates differs according to the species; many species are harmless commensals or endosymbionts of hosts including human. Otitis externa can cause considerable pain, distress and discomfort to the affected dog. The difficulty in treating O. externa is due to the complexity and multiple etiological agents and emergence of drug resistance. This study aimed to isolate pathogenic Candida spp. from dogs with O. externa infection and identification of the pathogen by routine Mycological, biochemical tests and molecular typing by using PCR as a rapid confirmatory test for identification the main Candida spp.